Journal: Scientific Reports

Article Title: Porcine CXCR1/2 antagonist CXCL8 (3–72) G31P inhibits lung inflammation in LPS-challenged mice

doi: 10.1038/s41598-020-57737-w

Figure Lengend Snippet: Recombinant PCXCL8 (3–72) N11R/G31P and PCXCL8 (3–72) G31P protein expression. The gene of porcine CXCL8 (3–72) N11R/G31P and CXCL8 (3–72) G31P were cloned into PGEX 6P-1, and then transformed into E. coli Rosetta (DE3) ( a–c ). Recombinant proteins CXCL8 (3–72) N11R/G31P (pN11R) and CXCL8 (3–72) G31P (pG31P) were induced by IPTG, treated with Prescission Protease, and then purified through GSTrap TM FF. Two proteins molecular weight of 10KD were finally obtained ( d ). The recombinant proteins could be identified by anti-human CXCL8 antibody ( e ).

Article Snippet: The membrane was blocked with 5% skim milk (Sigma, St. Louis, MO, U.S.A.), incubated in mouse anti-human CXCL8 antibody (CSB-PA08327A0Rb, CUSABIO, Wuhan, China) and HRP-conjugated goat-anti-rabbit IgG (CUSABIO, Wuhan, China), and visualized by ECL Western Blotting Substrate (Thermo Fisher, Waltham, MA, U.S.A.).

Techniques: Recombinant, Expressing, Clone Assay, Transformation Assay, Purification, Molecular Weight

Journal: Scientific Reports

Article Title: Porcine CXCR1/2 antagonist CXCL8 (3–72) G31P inhibits lung inflammation in LPS-challenged mice

doi: 10.1038/s41598-020-57737-w

Figure Lengend Snippet: pN11R and pG31P reduce inflammatory factors expression. LPS-challenged mice (LPS + saline) appeared increasing TNF-α, IL-1β and CXCL8 expression compared to saline-challenged mice. The expressions of TNF-α, IL-1β and CXCL8 were also enhanced after treated with pCXCL8 (LPS + pCXCL8), inversely dramatically reduced after treated with pN11R (LPS + pN11R) and pG31P (LPS + pG31P). *P < 0.05, **P < 0.01, ***P < 0.001, unpaired t-test, two-tailed.

Article Snippet: The membrane was blocked with 5% skim milk (Sigma, St. Louis, MO, U.S.A.), incubated in mouse anti-human CXCL8 antibody (CSB-PA08327A0Rb, CUSABIO, Wuhan, China) and HRP-conjugated goat-anti-rabbit IgG (CUSABIO, Wuhan, China), and visualized by ECL Western Blotting Substrate (Thermo Fisher, Waltham, MA, U.S.A.).

Techniques: Expressing, Saline, Two Tailed Test

Journal: Scientific Reports

Article Title: Porcine CXCR1/2 antagonist CXCL8 (3–72) G31P inhibits lung inflammation in LPS-challenged mice

doi: 10.1038/s41598-020-57737-w

Figure Lengend Snippet: Primers used for Real-Time PCR.

Article Snippet: The membrane was blocked with 5% skim milk (Sigma, St. Louis, MO, U.S.A.), incubated in mouse anti-human CXCL8 antibody (CSB-PA08327A0Rb, CUSABIO, Wuhan, China) and HRP-conjugated goat-anti-rabbit IgG (CUSABIO, Wuhan, China), and visualized by ECL Western Blotting Substrate (Thermo Fisher, Waltham, MA, U.S.A.).

Techniques: Sequencing